Pathology

Resources

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The TCGB occupies approximately 2,568 square feet of laboratory space on the third floor of the Center for Health Sciences, CHS 38-123, 38-126 and 33-388. It is operated by seventeen experienced genomic scientists. The TCGB is a high throughput genomic center utilizing the equipment listed below. In addition to offering wet-lab services, the TCGB has developed a broad informatics infrastructure and an efficient means for data acquisition, storage, and analysis. We have access to advanced software including Partek flow, VarSeq, and Falcon automated system, which significantly enhance the overall capacity of TCGB to rapidly process and transform the large quantity of data into novel findings.

Brief description of UCLA Technology Center for Genomics & Bioinformatics

Equipment

Next-Generation Sequencing

  • OxfordNANOPORE GriDIONx5 Sequencing System
  • 10X Genomics Chromium™ Controller Single Cell Sequencing System
  • 10x Genomics Chromium Connect
  • GeoMx DSP (Nanostring)
  • CosMx SMI (NanoString)
  • 10X Visium
  • Visium HD
  • Xenium
  • StereoSeq
  • NovaSeqTM X Plus Sequencing System
  • Illumina NextSeq 500 Sequencing System
  • Illumina MiSeq Personal Sequencer System
  • IntegenX Apollo 324 for automated NGS library preparation
  • Beckman Coulter BioMek i7 for fully automated workstation
  • Fluidigm Access Array System for target-enrichment
  • Covaris M220 for DNA shearing

Nucleic Acid Isolation

  • Roche MagNa Lyzer instrument for automated tissue homogenization
  • Biochain AnaPrep Automated Nucleic Acid Preparation System
  • Qiagen QIAcube HT Automated mid- to high-throughput Nucleic Acid Purification System

Quality Control

  • Agilent 2100 Bioanalyzer
  • Agilent 2200 Tapestation
  • NanoDrop 8000
  • Qubit 3.0 Fluorometer
  • Fragment Analyzer
  • Varioskan Lux Plate Reader

Other

  • Applied Biosystems 7500 Fast Real-Time PCR System
  • Bio-Rad C1000 Touch Thermal Cycler
  • Thermo Scientific DNA120 Speedvac Concentrator

Data Analysis (Software) / Other Resources

  • Partek flow
  • VarSeq
  • Falcon automated genomic analysis system
  • Hoffman2 data analysis clusters
  • Illumina base space Sequencing Hub professional
  • Amazon cloud HIPAA compliant research computing and storage system